期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 25, 页码 7131-7135出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201602239
关键词
excimer; fluorescence; imaging agents; photophysics; pi conjugation
资金
- CREST, JST
- Japan Advanced Plant Science Network
- World Premier International Research Center (WPI) Initiative, Japan
- Grants-in-Aid for Scientific Research [15K05385, 15F15778, 15H01083, 15H05482] Funding Source: KAKEN
Bright fluorescent molecules with long fluorescence lifetimes are important for the development of lifetime-based fluorescence imaging techniques. Herein, a molecular design is described for simultaneously attaining long fluorescence lifetime (tau) and high brightness (Phi(F) x epsilon) in a system that features macrocyclic dimerization of fluorescent p-conjugated skeletons with flexible linkers. An alkylene-linked macrocyclic dimer of bis(thienylethynyl)anthracene was found to show excimer emission with a long fluorescence lifetime (tau approximate to 19 ns) in solution, while maintaining high brightness. A comparison with various relevant derivatives revealed that the macrocyclic structure and the length of the alkylene chains play crucial roles in attaining these properties. In vitro time-gated imaging experiments were conducted as a proof-of-principle for the superiority of this macrocyclic fluorophore relative to the commercial fluorescent dye Alexa Fluor 488.
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