期刊
BIOMEDICAL OPTICS EXPRESS
卷 10, 期 4, 页码 1978-1998出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.10.001978
关键词
-
资金
- Gordon and Betty Moore Foundation's Data-Driven Discovery Initiative [GBMF4562]
- Ruth L. Kirschstein National Research Service Award [F32GM129966]
High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use speckle structured illumination microscopy (SIM) as a robust and cost-effective solution for high-content fluorescence microscopy with simultaneous high-content quantitative phase (QP). This multi-modal compatibility is essential for studies requiring cross-correlative biological analysis. Our method uses laterally-translated Scotch tape to generate high-resolution speckle illumination patterns across a large FOV. Custom optimization algorithms then jointly reconstruct the sample's super-resolution fluorescent (incoherent) and QP (coherent) distributions, while digitally correcting for system imperfections such as unknown speckle illumination patterns, system aberrations and pattern translations. Beyond previous linear SIM works, we achieve resolution gains of 4x the objective's diffraction-limited native resolution, resulting in 700 nm fluorescence and 1.2 mu m QP resolution, across a FOV of 2 x 2.7 mm(2), giving a space-bandwidth product (SBP) of 60 megapixels. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据