期刊
BIOMEDICAL OPTICS EXPRESS
卷 10, 期 4, 页码 1567-1580出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.10.001567
关键词
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资金
- National Institutes of Health [R33CA204582, R01EY028666, R01HD095520, U01CA202177]
- National Science Foundation [CMMI-1537027]
- NCI-UMD Partnership for Integrative Cancer Research
- University of Maryland's Brain and Behavior Initiative
In Brillouin microscopy, absorption-induced photodamage of incident light is the primary limitation on signal-to-noise ratio in many practical scenarios. Here we show that 660 nm may represent an optimal wavelength for Brillouin microscopy as it offers minimal absorption-mediated photodamage at high Brillouin scattering efficiency and the possibility to use a pure and narrow laser line from solid-state lasing medium. We demonstrate that live cells are similar to 80 times less susceptible to the 660 nm incident light compared to 532 nm light, which overall allows Brillouin imaging of up to more than 30 times higher SNR. We show that this improvement enables Brillouin imaging of live biological samples with improved accuracy, higher speed and/or larger fields of views with denser sampling. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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