期刊
ONCOTARGETS AND THERAPY
卷 12, 期 -, 页码 1447-1454出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S173207
关键词
non-small cell lung cancer; IL-27; miR-935
资金
- National Nature Science Foundation of China [81670024]
Introduction: Non-small-cell lung cancer (NSCLC) accounts for more than half of all lung cancer cases. Cytokines play an important role in NSCLC, including IL-27. IL-27 inhibits NSCLC progression; however, the mechanism is not clear. The purpose of this study is to investigate the effects of IL-27 on NSCLC cell proliferation and metastasis. Materials and methods: NSCLC cells were treated with IL-27 or transfected with miR-935, and the cell proliferation was assayed by Cell Counting Kit-8 (CCK-8) and colony formation. Cell metastasis was analyzed by Transwell chamber system and wound healing assay. IL-27 protein in the medium was analyzed by ELISA. IL-27 mRNA expression was measured by quantitative reverse transcriptase-PCR. Results: We found that IL-27 played an inhibiting role in NSCLC cell proliferation and metastasis. The molecular mechanism of the suppressing role of IL-27 in NSCLC was regulated by miR-935. IL-27 expression was negatively associated with miR-935 in the clinical NSCLC samples. Conclusion: The study revealed that IL-27 decreased lung cancer cell proliferation and metastasis via miR-935.
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