期刊
BMC NEPHROLOGY
卷 20, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12882-019-1221-5
关键词
Magnesium lithospermate B; Renal blood flow; Renal oxygen consumption; Hypoxia; CRF
资金
- National Natural Science Foundation of China General Projects [81573946]
- Scientific Research Foundation of Science and Technology Commission of Shanghai Municipal Government [16401931700]
- Scientific Research Foundation of Shanghai Municipal Commission of Health and Family Planning [201740193, 201540199]
- Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai [PWZxq2017-07]
- Science Foundation of Shuguang Hospital [SGKJ-201712]
Background: Magnesium lithospermate B (MLB) can promote renal microcirculation. The aim of the current project was to study whether MLB improves renal hemodynamics, oxygen consumption and subsequently attenuates hypoxia in rats induced by 5/6th renal Ablation/Infarction(A/I). Methods: Chronic renal failure (CRF) was induced in male SD rats by the 5/6 (A/I) surgery. 30 rats were randomly divided into three groups: sham group, 5/6 (A/I)+vehicle group (CRF group) and 5/6 (A/I)+MLB (CRF+MLB) group. 28days after the surgery, rats were given with saline or 100mg/kg MLB by i.p. injection for 8weeks. The 24-h urinary protein (24hUp), serum creatinine (Scr), blood urine nitrogen (BUN), systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured. The protein expression of Fibronectin (FN), Collagen-I (Col-I), Connective Tissue Growth Factor(CTGF) and Interleukin-6 (IL-6) were measured by Western blot. Renal blood flow (RBF) and renal O-2 consumption (QO(2)) indicated as sodium reabsorption (QO(2)/TNa) were detected before sacrifice. Renal hypoxia was assessed by measuring the protein expression of nNOS, HIF-1 alpha and VEGF. Results: MLB significantly reduced 24hUp, Scr, BUN, SBP and DBP levels in rats with CRF. The expression of FN, Col-I, CTGF and IL-6 were down-regulated by MLB treatment in rats with CRF. In comparison to sham operated rats, 5/6 (A/I) rats had significantly lower RBF, and MLB significantly increased RBF in rats with CRF. Moreover, QO(2)/TNa was higher in the CRF group as compared to that in the sham group, and it was significantly attenuated in the CRF+MLB group. MLB reversed the expression of nNOS (neuronal nitric oxide synthase), HIF-1 alpha (hypoxia inducible factor-1) and VEGF in rats with CRF. Conclusions: MLB improves renal function, fibrosis and inflammation in CRF rats induced by 5/6 (A/I), which is probably related to the increase in RBF, reduction of oxygen consumption and attenuation of renal hypoxia in the remnant kidney with CRF.
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