期刊
VIRUSES-BASEL
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/v11030303
关键词
La Crosse virus; C-type lectin receptor; innate immunity; mincle; CARD9; dendritic cells
类别
资金
- European Union [642870]
- German Research Platform for Zoonoses (GlycoViroLectinTools)
- Marie Curie Actions (MSCA) [642870] Funding Source: Marie Curie Actions (MSCA)
La Crosse virus (LACV) is a mosquito-transmitted arbovirus and the main cause of virus-mediated neurological diseases in children. To date, little is known about the role of C-type lectin receptors (CLRs)-an important class of pattern recognition receptors-in LACV recognition. DC-SIGN remains the only well-described CLR that recognizes LACV. In this study, we investigated the role of additional CLR/LACV interactions. To this end, we applied a flow-through chromatography method for the purification of LACV to perform an unbiased high-throughput screening of LACV with a CLR-hFc fusion protein library. Interestingly, the CARD9-associated CLRs Mincle, Dectin-1, and Dectin-2 were identified to strongly interact with LACV. Since CARD9 is a common adaptor protein for signaling via Mincle, Dectin-1, and Dectin-2, we performed LACV infection of Mincle(-/-) and CARD9(-/-) DCs. Mincle(-/-) and CARD9(-/-) DCs produced less amounts of proinflammatory cytokines, namely IL-6 and TNF-alpha, albeit no reduction of the LACV titer was observed. Together, novel CLR/LACV interactions were identified; however, the Mincle/CARD9 axis plays a limited role in early antiviral responses against LACV.
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