期刊
FOOD AND ENVIRONMENTAL VIROLOGY
卷 11, 期 1, 页码 40-51出版社
SPRINGER
DOI: 10.1007/s12560-019-09368-2
关键词
Viability RT-qPCR; Sodium deoxycholate; Infectivity; Drinking water; Inhibitory effects
资金
- Bureau of Waterworks Tokyo Metropolitan Government
- Health Labor Sciences Research Grant by the Ministry of Health, Labor and Welfare, Japan [H28-Kenki-Ippan-005]
The presence of pathogenic viruses in drinking water is a major public health concern. Although viability RT-qPCR methods were developed to quantify infectious viruses, they may not always reflect viral infectivity, therefore leading to false-positive results. In this study, sodium deoxycholate (SD) pre-treatment was used to improve the efficiency of viability RT-qPCR methods with respect to exclusive quantification of infectious viruses. The ability of SD pre-treatment to enhance the penetration of three viability markers, namely, ethidium monoazide (EMA, 100 mu M), propidium monoazide (PMA, 100 mu M), and cis-dichlorodiammineplatinum (CDDP, 1000 mu M), into heat-treated (90 degrees C for 1 min) Aichi virus at various concentrations (0.01-0.5%) was evaluated. The optimal SD concentration was found to be 0.1% for all markers. EMA/PMA/CDDP-RT-qPCR with 0.1% SD pre-treatment was significantly more effective than without SD pre-treatment in determining AiV inactivation after heat (50, 60, 70, 80, or 90 degrees C for 1 min) or chlorine treatment (1 mgCl(2)/L for 1, 2, 5, or 10 min). Among the viability RT-qPCR methods tested, CDDP-RT-qPCR with SD pre-treatment (SD-CDDP-RT-qPCR) was the most effective in reflecting viral infectivity. Performance testing of SD-CDDP-RT-qPCR in concentrated drinking water samples did not reveal any significant effects of SD-CDDP treatment. Thus, SD-CDDP-RT-qPCR could be a useful tool for monitoring infectious virus presence in drinking water.
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