期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 40, 页码 12494-12498出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201606742
关键词
DNA analysis; DNA sequencing; fragaceatoxins; nanopores; single-molecule studies
Nanopores are used in single-molecule DNA analysis and sequencing. Herein, we show that Fragaceatoxin C (FraC), an alpha-helical pore-forming toxin from an actinoporin protein family, can be reconstituted in sphingomyelin-free standard planar lipid bilayers. We engineered FraC for DNA analysis and show that the funnel-shaped geometry allows tight wrapping around single-stranded DNA (ssDNA), resolving between homopolymeric C, T, and A polynucleotide stretches. Remarkably, despite the 1.2 nm internal constriction of FraC, double-stranded DNA (dsDNA) can translocate through the nanopore at high applied potentials, presumably through the deformation of the alpha-helical transmembrane region of the pore. Therefore, FraC nanopores might be used in DNA sequencing and dsDNA analysis.
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