期刊
VIROLOGY
卷 528, 期 -, 页码 118-136出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2018.12.012
关键词
IBV; Lipid rafts; Clathrin mediated endocytosis; Endosome; Membrane fusion
类别
资金
- National Natural Science Foundation of China [31530074]
- Chinese Special Fund for Agricultural Sciences Research in the Public Interest [2014JB16, 2017JB05]
- China Ministry of Science and Technology [2017YFD0500802]
- Elite Youth Program of Chinese Academy of Agricultural Sciences [20170260401]
Although several reports suggest that the entry of infectious bronchitis virus (IBV) depends on lipid rafts and low pH, the endocytic route and intracellular trafficking are unclear. In this study, we aimed to shed greater light on early steps in IBV infection. By using chemical inhibitors, RNA interference, and dominant negative mutants, we observed that lipid rafts and low pH was indeed required for virus entry; IBV mainly utilized the clathrin mediated endocytosis (CME) for entry; GTPase dynamin 1 was involved in virus containing vesicle scission; and the penetration of IBV into cells led to active cytoskeleton rearrangement. By using R18 labeled virus, we found that virus particles moved along with the classical endosome/lysosome track. Functional inactivation of Rab5 and Rab7 significantly inhibited IBV infection. Finally, by using dual R18/DiOC labeled IBV, we observed that membrane fusion was induced after 1 h.p.i. in late endosome/lysosome.
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