期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 36, 页码 10626-10629出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201603731
关键词
biosensors; dynamic nuclear polarization; hyperpolarization; NMR spectroscopy; -glutamyl transpeptidase
资金
- CREST, the Japan Science and Technology Agency
- Centre d'Imagerie Biomedicale (CIBM) of the UNIL
- UNIGE
- HUG
- CHUV
- EPFL
- Swiss National Science Foundation [PPOOP1_157547]
- program Creation of Innovation Centers for Advanced Interdisciplinary Research Areas from JST
- JSPS
- Grants-in-Aid for Scientific Research [15J03449] Funding Source: KAKEN
The -glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, -Glu-[1-C-13]Gly, for monitoring GGT activity invivo by hyperpolarized (HP) C-13 magnetic resonance (MR). The properties of -Glu-[1-C-13]Gly are suitable for invivo HP C-13 metabolic analysis since the chemical shift between -Glu-[1-C-13]Gly and its metabolic product, [1-C-13]Gly, is large (4.3ppm) and the T-1 of both compounds is relatively long (30s and 45s, respectively, in H2O at 9.4T). We also demonstrate that -Glu-[1-C-13]Gly is highly sensitive to invivo modulation of GGT activity induced by the inhibitor acivicin.
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