期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 364, 期 -, 页码 29-44出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2018.12.004
关键词
Lung; Broccoli; Hyperoxia; Microarray; Antioxidant response element
资金
- Intramural Research Program of the NIEHS, National Institutes of Health, United States
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [ZICES102445, ZIAES102825, ZICES103005, ZIAES100475, ZIAES100513, ZIAES100557] Funding Source: NIH RePORTER
Nrf2 is essential to antioxidant response element (ARE)-mediated host defense. Sulforaphane (SFN) is a phytochemical antioxidant known to affect multiple cellular targets including Nrf2-ARE pathway in chemoprevention. However, the role of SFN in non-malignant airway disorders remain unclear. To test if pre-activation of Nrf2-ARE signaling protects lungs from oxidant-induced acute injury, wild-type (Nrf2(+/+)) and Nrf2-deficient (Nrf2(-/-)) mice were given SFN orally or as standardized broccoli sprout extract diet (SBE) before hyperoxia or air exposure. Hyperoxia-induced pulmonary injury and oxidation indices were significantly reduced by SFN or SBE in Nif2(+/+) mice but not in Nrf2(-/-) mice. SFN upregulated a large cluster of basal lung genes that are involved in mitochondrial oxidative phosphorylation, energy metabolism, and cardiovascular protection only in Nrf2(+/+) mice. Bioinformatic analysis elucidated ARE-like motifs on these genes. Transcript abundance of the mitochondrial machinery genes remained significantly higher after hyperoxia exposure in SFN-treated Nrf2+/+ mice than in SFN-treated Nrf2(-/-) mice. Nuclear factor-kappa B was suggested to be a central molecule in transcriptome networks affected by SFN. Minor improvement of hyperoxia-caused lung histopathology and neutrophilia by SFN in Nrf2(-/-) mice implies Nrf2-independent or alternate effector mechanisms. In conclusion, SFN
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