Opportunities and challenges related to the implementation of sperm cryopreservation into breeding of salmonid fishes

The main aim of the present review is to present the opportunities and challenges associated with the application of cryopreserved sperm, which may improve the breeding of salmonid fishes. Cryopreservation of sperm has been used as a strategy for the conservation of biodiversity of fishes populations, the preservation of sperm from the most valuable breeding individuals and facilitate transportation of genomes, and providing a biological source of sperm regardless of the synchronisation of the maturity of broodstocks. Cryopreserved sperm can be used for the genetic improvement of salmonid fishes based on the programs of individual crossing of selected males with individual females. However, these opportunities have not yet been fully implemented at the conditions of hatchery practice. Despite the significant progress concerning the standardization of cryopreservation procedures, there are still more challenges than opportunities related to the implementation of sperm cryopreservation into breeding of salmonid fishes. The main challenge concerns the scaling up of the method towards fulfilling the requirements of fishes-breeders, in particular mass production of eyed eggs and fry. The present review shows knowledge gaps that should be considered in further studies, including development of methods to obtain sufficient amounts of sperm from numerous species of salmonids, scaling up the methods towards cryopreservation of high volumes of sperm and towards thawing high number of straws, and optimizing artificial fertilization in which oocytes are fertilized with high numbers of frozen/thawed sperm. Moreover, the implementation of technologies into hatchery practice will require special consideration to counteract the risk of sperm infection and its transmission to offsprings during cryopreservation and storage in liquid nitrogen. (C) 2019 Elsevier Inc. All rights reserved.