期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 169, 期 2, 页码 374-381出版社
HUMANA PRESS INC
DOI: 10.1007/s12011-015-0402-8
关键词
Common carp; Subacute; Copper; Cadmium; Quantitative RT-PCR; Western blot
资金
- Postdoctoral Science Founds of Heilongjiang Province [LRB10-633]
- National Natural Science Foundation of China [31470131]
- National and Local Joint Freshwater Fish Breeding Engineering Laboratory of China
Copper (Cu) and cadmium (Cd) are the most common heavy metals that are easily detected in aquatic environments on a global scale. In this paper, we investigated the messenger RNA (mRNA) and protein levels of HSPs (HSP60, HSP70, and HSP90) in the liver of the common carp exposed to Cu, Cd, and a combination of both metals by real-time quantitative PCR and Western blot. The results indicated that in each exposure group, the mRNA levels of HSP60, HSP70, and HSP90 were increased significantly compared to the corresponding controls after 96 h of exposure (P < 0.05). A significant increase was observed in the HSP70 protein level in the high-dose Cu group and all of the Cd groups. Significant increases were also observed in the protein levels of HSP60 and HSP90 in the high combination group and the low combination group, respectively. These results indicated that the dynamics of HSP expression observed in the common carp support the role of HSPs as biochemical markers in response to environmental pollution and provided valuable insights into the adaptive mechanisms used by the common carp to adapt to the challenges of stressful environments.
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