期刊
STEM CELLS AND DEVELOPMENT
卷 28, 期 7, 页码 489-496出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2018.0104
关键词
immunomodulation; mesenchymal stem cells; facial nerve; motor neurons
资金
- National Natural Science Foundation of China [81470698]
- Beijing Natural Science Foundation [7172176]
- National Key Research and Development Plan of China [2016YFA0101000, 2016YFA0101002]
- Scientific Project of Young and Middle-aged Researcher of PUMCH [pumch-2013-007]
Normal facial nerve (FN) function is very important for human being. However, if injured, FN function is difficult to restore completely. Recently, many studies reported the immune regulation function of stem cells (SCs). However, the immunomodulation function of SCs on FN injury is still unclear. Our study aims to explore the mechanism of immunomodulation effect of Sprague-Dawley rat bone marrow-derived SCs (BMSCs) on FN injury and specially focus on the regulation of Th17 and the protection effects of BMSCs on central facial motor neurons (FMNs). First, rat FNs were harvested. FN and BMSCs were cultured together or separately and levels of transforming growth factor (TGF)-beta 1, interleukin (IL)-6, hepatocyte growth factor (HGF), inducible nitric oxide synthase (iNOS), and prostaglandin E2 (PGE2) in supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Then, after treating with or without local BMSCs injection, the proportion of Th17 in neck lymph nodes (LNs) was investigated in rat FN injury models. Furthermore, the apoptotic index of FMNs was studied in rat FN injury models that were treated with or without BMSCs. We found that BMSCs could secrete high levels of IL-6, HGF, PGE2, iNOS, and TGF-beta 1 in culture. The percentage of Th17 of neck LNs in BMSCs-treated group was significantly lower than that in the control group. The apoptotic index of FMNs in BMSCs-treated group was significantly lower than that in the control group. In conclusion, our research indicates BMSCs could independently secrete cytokines IL-6, HGF, PGE2, iNOS, and TGF-beta 1, and these cytokines could regulate the balance among subsets of CD4(+) T cells and could protect FMNs by inhibiting neuron apoptosis.
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