期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 210, 期 -, 页码 335-340出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.11.048
关键词
Split DNAzyme; TMB; Hemin-graphene composites; Colorimetry; T-Hg2+-T
类别
资金
- Chongqing Key Laboratory of Catalysis and New Environmental Materials [KFJJ2017033]
- Open Research Fund [CQCM-2016-05]
- Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [201712]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJ1706156]
- Project of Wenzhou Science & Technology Bureau [W20170006]
- Open Project of State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials [17kffk06]
- Startup Foundation of Chongqing Technology and Business University [1756001]
- National Natural Science Foundation of China [31300819]
A visual and sensitive Hg2+ detection strategy was developed based on split DNAzyme amplification and hemin-graphene oxide composites (H-GNs). Two split DNAzyme sequences can form two entire enzyme-strands DNA (E-DNA) by T-Hg2+-T interaction. The E-DNA can bind with the loop of molecular beacon (MB) to form Mg2+-dependent DNAzyme structure. The formed DNAzyme can circularly cleave the loop of MB, resulting large amount of DNA fragments. The resultant DNA fragments can prevent H-GNs from aggregation by adsorbing on its surface. Consequently, the supernate with large amount of H-GNs shows dark blue color after chromogenic reaction. This strategy shows a linear range from 50 pM to 1200 pM. The limit detection can be low to 33 pM. This strategy provides a visual and enzyme-free amplification mode for quick and sensitive screen of Hg2+. (C) 2018 Elsevier B.V. All rights reserved.
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