期刊
SMALL
卷 15, 期 12, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201804987
关键词
glucose oxidase; inorganic; protein hybrid; integrated enzyme cascade bioplatform; nanozymes; self-assembly
类别
资金
- National Nature Science Foundations of China [21573190]
- Nature Science Key Basic Research of Jiangsu Province for Higher Education [15KJA150009]
- PAPD
Nanozymes provide new opportunities for facilitating next generation artificial enzyme cascade platforms. However, the fabrication of high-performance integrated artificial enzyme cascade (IAEC) bioplatforms based on nanozymes remains a great challenge. A facile and effective self-assembly strategy for constructing an IAEC system based on an inorganic/protein hybrid nanozyme, beta-casein-BiPt nanochain@GO (CA-BiPtNC@GO) nanohybrid with unique physicochemical surface properties and hierarchical structures, is introduced here. Due to the synergetic effect of the protein, GO, and Bi3+, the hybrid acts as highly adaptable building blocks to immobilize natural enzymes directly and noncovalently without the loss of enzyme activity. Simultaneously, the CA-BiPtNC@GO nanohybrid exhibits outstanding peroxidase-mimicking activity and works well with natural oxidases, resulting in prominent activity in catalyzing cascade reactions. As a result, the proposed IAEC bioplatform exhibits excellent sensitivity with a wide linear range of 0.5 x 10(-6) to 100 x 10(-6)m and a detection limit of 0.05 x 10(-6)m for glucose. Meticulous design of ingenious hierarchically nanostructured nanozymes with unique physicochemical surface properties can provide a facile and efficient way to immobilize and stabilize nature enzymes using self-assembly instead of chemical processes, and fill the gap in developing robust nanozyme-triggered IAEC systems with applications in the environment, sensing, and synthetic biology.
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