期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 290, 期 -, 页码 203-209出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.03.121
关键词
Zinc ions; Electrochemiluminescence; Palladium nanocubes; Porphyrin
资金
- National Natural Science Foundation of China [21475118, 21675093]
- Natural Science Foundation of Zhejiang province [LQ19B050004]
- Open Research Fund of Key Laboratory of the Ministry of Education for Advanced Catalysis Materials (Zhejiang Normal University)
- PhD Fund of ZJNU for Young Teachers [zz323205020517002104]
- Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology [SATM201703, STAM201804]
- Zhejiang province of undergraduate scientific and technological innovation project (New Talents Program) [2018R404025]
Zinc ions (Zn2+) act as a transcription factor in eukaryotic cells for mediating protein-protein interactions in human healthy. The simple and sensitive detection of Zn2+ is the crucial mission in analytical field. This work demonstrated a novel and fast Zn2+ detection strategy based on signal on mode with electrochemiluminescence (ECL) luminophore, in which singlet oxygen was generated via electrocatalytic reduction of zinc proto-porphyrin IX grafted onto Pd nanocubes (ZnPPIX-Pd NCs). In protocol, Pd NCs were synthetized under hydrothermal conditions and aminated through thermal oxidative polymerization of 3-aminopropyltriethoxysilane (APTES), followed by further linking with PPIX via amide reaction. The resulting PPIX-Pd NCs effectively captured Zn2+ in chelation and monitored by Fourier transform infrared (FTIR) spectroscopy and ultraviolet-visible (UV-vis) absorption spectroscopy. By virtue of the metal resonance responses of Pd NCs, an intense monochromic, potential shuffling and enhanced ECL irradiation shows up for the hybrid nanocomposite with H2O2 as the coreactant. To this end, an ultrasensitive method based on the nanocomposite with a signal-on model was developed for Zn2+ detection with a low detection limit down to 0.38 nM (based on a S/N = 3), excellent precision and high stability. The ECL strategy reveals the potential application in molecular diagnosis and offers a new avenue for bioassay of structural Zn(II) proteins and zinc finger-binding nucleotides.
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