期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 281, 期 -, 页码 150-156出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.10.080
关键词
Plum Pox Virus; Sharka; EGOFET; Organic bioelectronics
资金
- Fondazione di Vignola
- Life Science Department through FAR2017
Plum Pox Virus (PPV) is the pathogen responsible for Sharka, a highly infectious disease affecting stone fruit trees and causing severe economic damages, which can be only contained through early-detection and frequent monitoring. We propose a bioelectronic PPV biosensor, based on a electrolyte-gated organic field-effect transistor (EGOFET), for the specific detection of PPV in plant extracts with a sub ng/ml detection limit. The sensing unit of the biosensor is based on anti-PPV antibodies, uniformly oriented on the gold gate electrode by using a sub-monolayer of Protein G. The sensitivity and dynamic range of the EGOFET-based biosensor are comparable to those of commercially available platforms for detection of plant pathogens. This novel electronic immunosensor is compatible with low-cost fabrication procedures and can be easily reconfigured into a fully portable device to be operated in greenhouse and in the field orchards.
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