期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 280, 期 -, 页码 41-45出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.10.044
关键词
Tyrosinase; Lucigenin; Kojic acid; Fluorescent biosensor; Inhibitors; Drug screening
资金
- National Natural Science Foundation of China [21475123, 21675148]
- National Key Research and Development Program of China [2016YFA0201300]
- Chinese Academy of Sciences (CAS)-the Academy of Sciences for the Developing World (TWAS) President's Fellowship Program
Tyrosinase is a key enzyme in melanin biosynthesis and its serum level is an important biomarker for some dermatoses such as vitiligo and melanoma. Thus, a sensitive and facile assay for tyrosinase activity is in urgent demands. Lucigenin is a well-known chemiluminescent luminophore, but has been rarely been employed for fluorescence detection. In this work, a facile method to detect tyrosinase activity based on lucigenin fluorescence has been reported for the first time. When tyrosinase was mixed with tyrosine, tyrosine was oxidized to generate melanin-like polymers which can effectively quench the fluorescence of lucigenin. A linear range of tyrosinase from 0.25 to 25 mu g/mL was obtained with detection limit of 0.2 mu g/mL. In addition, a useful platform for screening potential tyrosinase inhibitors was constructed based on the proposed system. The linear range is from 2.5 to 62.5 mu g/mL and the detection limit is as low as 1.0 mu g/mL for kojic acid, a typical tyrosinase inhibitor.
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