期刊
SENSORS
卷 19, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/s19051234
关键词
lensless holographic microscopy; LED; Complementary Metal-Oxide Semiconductor (CMOS) image sensor; cell culture; cell imaging; cell counting
资金
- Lower Saxony Ministry for Science and Culture (N-MWK), Germany
- European Union's Horizon 2020 research and innovation program [737089]
- European Union's Seventh Framework Program (FP/2007-2013)/ERC [336917]
- Serra Hunter Program
- ICREA Academia Program
- DFG Project GrK NanoMet
- German Research Foundation
- Open Access Publication Funds of the Technische Universitat Braunschweig
Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.
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