期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 671, 期 -, 页码 850-865出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2019.03.115
关键词
Selenium; Mitochondria; Oxidative stress; Gene expression; Tetrahymena thermophila
资金
- MINECO [CGL2016-75494-R]
Selenium is an essential micronutrient but at high concentrations can produce severe cytotoxicity and genomic damage. We have evaluated the cytotoxicity, ultrastructural and mitochondrial alterations of the two main selenium inorganic species; selenite and selenate, in the eukaryotic microorganism Tetrahymena thermophila. In this ciliate, selenite is more toxic than selenate. Their LC50 values were calculated as 27.65 mu M for Se(IV) and 56.88 mM for Se (VI). Significant levels of peroxides/hydroperoxides are induced under low-moderate selenite or selenate concentrations. Se(VI) exposures induce an immediate mitochondrial membrane depolarization. Seleniumtreated cells show an intense vacuolization and some of them present numerous discrete and small electrondense particles, probably selenium deposits. Mitochondrial fusion, an intense swelling in peripheral mitochondria and mitophagy are detected in selenium treated cells, especially in those exposed to Se (IV). qRT-PCR analysis of diverse genes, encoding relevant antioxidant enzymes or other proteins, like metallothioneins, involved in an environmental general stress response, have shown that they may be crucial against Se(IV) and/or Se (VI) cytotoxicity. (C) 2019 Elsevier B.V. All rights reserved.
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