期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 169, 期 2, 页码 247-253出版社
HUMANA PRESS INC
DOI: 10.1007/s12011-015-0417-1
关键词
AlCl3; Osteoblast apoptosis; BAPTA-AM; Calcium homeostasis; Ca2+/CaMKII signal pathway
资金
- National Science Foundation [31372375]
- Science and Technology Program of Heilongjiang Educational Bureau [C201425]
Aluminum promotes osteoblast (OB) apoptosis. Apoptosis is induced by the disordered calcium homeostasis. Therefore, to investigate the relationship between Al-induced OB apoptosis and calcium homeostasis, calvarium OBs from neonatal rats (3-4 days) were cultured and exposed to 0.048-mg/mL Al3+ or 0.048-mg/mL Al3+ combined with 5 mu M BAPTA-AM (OBs were pretreated with 5 mu M BAPTA-AM for 1 h, then added 0.048 mg/mL Al3+), respectively. Then OB apoptosis rate, intracellular calcium ions concentration ([Ca2+]i), mRNA expression level of calmodulin (CaM), and protein expression levels of CaM and p-CaMKII in OBs were examined. The result showed that AlCl3 increased OB apoptosis rate, and [Ca2+]i and p-CaMKII expression levels and decreased CaM expression levels, whereas BAPTA-AM relieved the effects. These results proved that AlCl3 induced OB apoptosis by disrupting the intracellular Ca2+ homeostasis and activating the Ca2+/CaMKII signal pathway. Our findings can provide new insights for revealing the apoptosis mechanism of OBs exposed to AlCl3.
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