期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 651, 期 -, 页码 2424-2431出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2018.10.079
关键词
Endocrine disruption; Pyrethroid pesticides; Aquatic toxicology; ERa knockdown; Developmental toxicology
资金
- CAPES Foundation, Ministry of Education of Brazil, through the program Science Without Borders [BEX 99999.013554/2013-01]
- AES/USDA Resource Allocation Program at the University of California, Riverside
- NRSA T32 Training Program [T32ES018827]
- USDA National Institute of Food and Agriculture Hatch Project [1009609]
Bifenthrin (BF) is a pyrethroid insecticide widely used in urban and agricultural applications. Previous studies in embryos of zebrafish have shown that BF can affect estradiol biosynthesis and the dopaminergic system. To examine the role of the estrogen receptor (ER) in the endocrine effects of BF, embryos were exposed for 96 h to a mixture of 0.15 and 1.5 mu g/L BF and an ER agonist (17 alpha-ethynylestradiol - EE2) at 0.09 mu g/L. Transcripts related to estrogenic (vitellogenin VTG) and dopaminergic (tyrosine hydroxylase (TH), dopamine receptor 1 (DR1), monoamine oxidase (MAO), and catechol-O-methyltransferase b (COMTb)) signaling pathways were investigated by qRT-PCR. Dopamine (DA) and itsmetabolites (homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC)) were also measured. There was a significant increase in VTG, DR1, MAO and COMTbmRNA levels and HVA-DA ratios within all zebrafish embryos exposed to EE2, including EE2 alone, 0.15 mu g/L BF + EE2 and 1.5 mu g/L BF + EE2. A significant decrease in homogenate concentrations of DA was observed within all zebrafish embryos exposed to EE2, which included EE2 alone, 0.15 mu g/L BF + EE2 and 1.5 mu g/L BF + EE2. Co-exposure of BF with EE2 failed to diminish estrogenic or dopaminergic signaling in embryos. Additionally, embryos with diminished ERa expression by morpholino injection were exposed to 0.15 mu g/L BF, 1.5 mu g/L BF and 0.09 mu g/L EE2, with subsequent gene expression measurements. ER alpha knockdown did not prevent the effects of BF, indicating ER alpha may have a limited role in the estrogenic and dopaminergic effects caused by BF in zebrafish embryos. (C) 2018 Elsevier B.V. All rights reserved.
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