期刊
RNA BIOLOGY
卷 18, 期 2, 页码 168-177出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2019.1582955
关键词
La protein; La – related protein; RNA binding protein; precursor tRNA; RNA processing; protein domain; RNA-protein interaction; translational control; RNA chaperone
资金
- CIHR Funding Source: Medline
La protein shuttles between the nucleus and cytoplasm to bind nascent RNA polymerase III transcripts and mRNAs using different binding modes, protecting RNA precursors and promoting maturation and translation. Studies have shown that La proteins utilize multiple binding modes beyond the well-characterized UUU-3' end recognition, expanding our understanding of their RNA chaperone activity.
La shuttles between the nucleus and cytoplasm where it binds nascent RNA polymerase III (pol III) transcripts and mRNAs, respectively. La protects the 3MODIFIER LETTER PRIME end of pol III transcribed RNA precursors, such as pre-tRNAs, through the use of a well-characterized UUU-3MODIFIER LETTER PRIMEOH binding mode. La proteins are also RNA chaperones, and La-dependent RNA chaperone activity is hypothesized to promote pre-tRNA maturation and translation at cellular and viral internal ribosome entry sites via binding sites distinct from those used for UUU-3MODIFIER LETTER PRIMEOH recognition. Since the publication of La-UUU-3MODIFIER LETTER PRIMEOH co-crystal structures, biochemical and genetic experiments have expanded our understanding of how La proteins use UUU-3MODIFIER LETTER PRIMEOH-independent binding modes to make sequence-independent contacts that can increase affinity for ligands and promote RNA remodeling. Other recent work has also expanded our understanding of how La binds mRNAs through contacts to the poly(A) tail. In this review, we focus on advances in the study of La protein-RNA complex surfaces beyond the description of the La-UUU-3MODIFIER LETTER PRIMEOH binding mode. We highlight recent advances in the functions of expected canonical nucleic acid interaction surfaces, a heightened appreciation of disordered C-terminal regions, and the nature of sequence-independent RNA determinants in La-RNA target binding. We further discuss how these RNA binding modes may have relevance to the function of the La-related proteins.
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