Pentacyclic triterpenes and other constituents in propolis extract from Melipona beecheii collected in Yucatan, Mexico

Thirteen pentacyclic triterpenes, methyl 3-oxours-12-en-23-oate, marsformosanone, taraxerone, beta-amyrenone, alpha-amyrenone, lupenone, 24-methylencycloartan-3-one, moretenol acetate, beta-amyrin acetate, germanicol acetate, 24-methylencycloartanyl acetate, beta-amyrin, and alpha-amyrin were identified in a chloroform-methanol propolis extract from Melipona beecheii. Additionally, were identified in this propolis, hexadecanoic acid, methyl ester, octadecanoic acid, methyl ester and 1-triacontanol. The purification of the propolis extract was carried out using different chromatographic techniques, including vacuum liquid chromatography, gravity column chromatography and gel filtration chromatography Sephadex LH-20. The identification of the metabolites was performed using mass spectrometry. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.