期刊
PSYCHONEUROENDOCRINOLOGY
卷 106, 期 -, 页码 77-84出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.psyneuen.2019.03.019
关键词
Oxytocin; Chemogenetics; Ca2+ imaging; Microdialysis; DREADD; Social behavior; Emotional behavior; AAV
资金
- Deutsche Forschungsgemeinschaft (DFG) [GRK 2174, NE465/27-1]
- BMBF [FKZ 01EE1401A]
- EU [FemNAT-CD 930742]
- DFG within the Collaborative Research Center (SFB) [1134, 1158]
- ANR-DFG [GR 3619/701]
- Chica and Heinz Schaller Research Foundation
- Human Frontier Science Program [RGP0019/2015]
- SNSF-DFG [GR 3619/8-1]
Chemogenetics provides cell type-specific remote control of neuronal activity. Here, we describe the application of chemogenetics used to specifically activate oxytocin (OT) neurons as representatives of a unique class of neuroendocrine cells. We injected recombinant adeno-associated vectors, driving the stimulatory subunit hM3Dq of a modified human muscarinic receptor into the rat hypothalamus to achieve cell type-specific expression in OT neurons. As chemogenetic activation of OT neurons has not been reported, we provide systematic analysis of the temporal dynamics of OT neuronal responses in vivo by monitoring calcium fluctuations in OT neurons, and intracerebral as well as peripheral release of OT. We further provide evidence for the efficiency of chemogenetic manipulation at behavioral levels, demonstrating that evoked activation of OT neurons leads to social motivation and anxiolysis. Altogether, our results will be profitable for researchers working on the physiology of neuroendocrine systems, peptidergic modulation of behaviors and translational psychiatry.
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