期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 15, 页码 7323-7332出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1814965116
关键词
chromatin organization; fluorescence lifetime imaging microscopy; DNA repair; spatiotemporal correlation spectroscopy; Forster resonance energy transfer
资金
- Cancer Council NSW
- Australian National Health and Medical Research Council (NHMRC)
- Australian NHMRC [APP1059278, 1053195, 1106241, 1104461, APP1104461, APP1124762]
- Australian Research Council (ARC) [CE140100011, LP140100967]
- National Institutes of Health [P41-GM103540, P50-GM076516]
- Cancer Council NSW [RG 15-12]
- Cancer Institute NSW [11/FRL/5-02]
- ARC [DP180101387]
- National Health and Medical Research Council of Australia [1106241, 1104461] Funding Source: NHMRC
To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we established a biophysical method to quantify chromatin compaction at the nucleo-some level during the DNA damage response (DDR). The method is based on phasor image-correlation spectroscopy of histone fluorescence lifetime imaging microscopy (FLIM)-Forster resonance energy transfer (FRET) microscopy data acquired in live cells coex-pressing H2B-eGFP and H2B-mCherry. This multiplexed approach generates spatiotemporal maps of nuclear-wide chromatin compaction that, when coupled with laser microirradiation-induced DSBs, quantify the size, stability, and spacing between compact chromatin foci throughout the DDR. Using this technology, we identify that ataxia-telangiectasia mutated (ATM) and RNF8 regulate rapid chromatin decompaction at DSBs and formation of compact chromatin foci surrounding the repair locus. This chromatin architecture serves to demarcate the repair locus from the surrounding nuclear environment and modulate 53BP1 mobility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据