期刊
ANALYTICAL METHODS
卷 8, 期 22, 页码 4319-4323出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay00900j
关键词
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资金
- National Natural Science Foundation of China [21275096, 21475083]
- Program for Innovative Research Team in Shaanxi Province [2014KCT-28]
Sensitive detection of uracil-DNA glycosylase (UDG) activity is critical for the functional study of UDG and clinical diagnosis. Herein we exploit dsDNA-templated copper nanoclusters (CuNCs) as a fluorescent nano-dye for UDG assay. The UDG-catalyzed uracil removal can liberate ssDNA from the uracil-containing dsDNA substrate, and thus the introduction of Cu2+ and ascorbate cannot form the fluorescent CuNCs due to the lack of the dsDNA templates. By optimizing the number and location of uracil on the DNA strand, a low detection limit of 0.0005 UmL(-1) was achieved. The simple and sensitivemethodmay hold potential applications in UDG-related clinical diagnosis and functional research.
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