期刊
PLOS ONE
卷 14, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0211941
关键词
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资金
- University Nursing Programs for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2017003]
- Young Talents Project of Northeast Agricultural University [17QC13]
The anionic peroxidases play an important role in a variety of plant physiological processes. We characterized and isolated the Zmap promoter (PZmap) at the 50 flanking region in order to better understand the regulatory mechanisms of Zmap gene expression. A series of PZmap deletion derivatives, termed a1-a6, at positions -1694, -1394, -1138, -784, -527 and -221 from the translation start site were blended to the beta-glucuronidase reporter gene. Agrobacterium-mediated transformation method was used to study each deletion construct in tobaccos. Sequence analysis showed that several cis-acting elements (MYB binding site, Box-II, a TGACG-element, a CGTCA-element and a low temperature responsive element) were located within the promoter. Deletion analysis suggested the sequence between -1,694 and -1394bp may contain cis-elements associated with GUS up regulation. The MYB binding site (-757) might act as a negative drought-responsive element. There might be repressor elements located in the region (-1,694 to -1394bp) to repress Zmap expression under 4 degrees C. The characterized promoter would be an ideal candidate for genetic engineering for improving the resistance of maize to different stressors.
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