Integration of RACK1 and ethylene signaling regulates plant growth and development in Arabidopsis

Arabidopsis RACK1 (Receptors for Activated C Kinase 1) are versatile scaffold proteins that have been shown to be involved in the regulation of plant response to plant hormones including auxin, ABA, gibberellin and brassinosteroid, but not ethylene. By characterizing the double and triple mutants of RACK1 genes, we found that rack1 mutants showed reduced sensitivity to ethylene. By characterizing double and high order mutants generated between ein2, a loss-of-function mutant of the key ethylene signaling regulator gene EIN2 (Ethylene INsensitive 2), and rack1 mutants, we found that loss-of-function of EIN2 partially recovered some phenotypes observed in the rack1 mutants, such as low-fertility and reduced root length and rosette size. On the other hand, the ein2 rack1 mutants produced more rosette leaves, and flowered late when compared with ein2 and the corresponding rack1 mutants. We also found that the curled leaves and twisted petioles phenotypes observed in the ein2 mutants were enhanced in the ein2 rack1 mutants. However, assays in yeast indicated that EIN2 may not physically interact with RACK1. On the other hand, RT-PCR results showed that the expression level of EIN2 was reduced in the rack1 mutants. Taken together, our results suggest that RACK1 may integrate ethylene signaling to regulate plant growth and development in Arabidopsis.