期刊
PLANT SCIENCE
卷 280, 期 -, 页码 297-304出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2018.12.020
关键词
Cyclin; Cyclin dependent kinase (CDK); Germination; KRP; Maize; PCNA
资金
- Consejo Nacional de Ciencia y Tecnologia [CB 220661]
- Programa de Apoyo a Proyectos de Investigation e Innovation Tecnologica [IN215316, IA204817]
- Programa de Apoyo a Investigation y Posgrado [5000-9124, 5000-9130]
- Consejo Nacional de Ciencia y Tecnologia
- Research Assistant FQ-UNAM grant
The Proliferating Cell Nuclear Antigen, PCNA, has roles in both G1 and S phases of the cell cycle. Here we show that maize PCNA can be found in cells in structures of a trimer or a dimer of trimer, in complexes of high molecular mass that change in size as germination proceeds, co-eluting with cell cycle proteins as CycD3;1 and CDKs (A/B1;1). Using different methodological strategies, we show that PCNA actually interacts with CycD3;1, CDKA, CDKB1;1, KRP1;1 and KRP4;1, all of which contain PIP or PIP-like motifs. Anti-PCNA immunoprecipitates show kinase activity that is inhibited by KRP1;1 and KRP4;2, indicating the formation of quaternary complexes PCNA-CycD/CDKs-KRPs in which PCNA would act as a platform. This inhibitory effect seems to be differential during the germination process, more pronounced as germination advances, suggesting a complex regulatory mechanism in which PCNA could bind different sets of cyclins/CDKs, some more susceptible to inhibition by KRPs than others.
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