期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 137, 期 2, 页码 297-308出版社
SPRINGER
DOI: 10.1007/s11240-019-01571-0
关键词
Bupleurum chinense DC; bZIP transcription factor BcbZIP134; Hairy root; Overexpression; Biosynthesis of saikosaponin
资金
- National Natural Science Foundation of China [81773847]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2M-2-003]
- National Standardization Project of Traditional Chinese Medicine [ZYBZH-Y-JIN-34]
- Sichuan Province 13th FiveYear Crop and Livestock Breeding Key Varieties Breeding Projects [2016NYZ0020]
Chaihu, the dried roots of several Bupleurum species, are widely used as herbal drugs. Triterpene saponins, as saikosaponins (SSs) in Chaihu, exhibit a wide range of pharmacological actions, including significant anti-inflammatory, immunomodulatory, hepatoprotective, antioxidant, anti-tumor and antiviral activities. To explore the regulatory biosynthesis of SS in Bupleurum chinense roots, we screened putative transcription factor (TF) genes from transcriptome data. Here, we report one of the bZIP TF genes, named BcbZIP134, which belongs to bZIP G subfamily. The expression of BcbZIP134 was elevated in MeJA-treated adventitious roots of B. chinense. No obvious differential expression of BcbZIP134 was detected in tissues (root, stem, leaf, flower and fruit) of B. chinense. Overexpressing BcbZIP134 in B. chinense hairy roots revealed that the content of the main SS monomers a, c and d were decreased compared to control hairy roots. Furthermore, the contents of a few other minor SS monomers were also affected when compared to the controls. The results indicate that BcbZIP134 may play a negative regulatory role in SS biosynthesis. Key MessageChaihu, the dried roots of several Bupleurum species, are successfully and widely used as herbal drugs. Triterpene saponins, as saikosaponins (SSs) in Chaihu, exhibit a wide range of pharmacological actions, including significant anti-inflammatory, immunomodulatory, hepatoprotective, antioxidant, anti-tumor and antiviral activities. To explore the regulatory biosynthesis of SS in B. chinense roots, we screened putative transcription factor (TF) genes from transcriptome data. Here, we report one of the bZIP TF genes, named BcbZIP134, which encodes a predicted 410 amino acid protein belonging to bZIP G subfamily. The expression of BcbZIP134 was elevated in MeJA-treated adventitious roots of B. chinense, and no obvious differential expression of BcbZIP134 was detected in tissues (root, stem, leaf, flower and fruit) of B. chinense. Overexpressing BcbZIP134 in B. chinense hairy roots revealed that the content of the main SS monomers a, c and d were decreased compared to control hairy roots. Furthermore, the contents of a few other minor SS monomers were also affected when compared to the controls. The results indicate that BcbZIP134 may play a negative regulatory role in SS biosynthesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据