期刊
PLANT AND SOIL
卷 438, 期 1-2, 页码 39-55出版社
SPRINGER
DOI: 10.1007/s11104-019-03992-4
关键词
Abscisic acid (ABA); Alkaline stress; Priming; Reactive oxygen species (ROS); Rice (Oryza sativa L.)
资金
- National Key Research and Development Program of China [SQ2018YFD020224]
- Chinese Academy of Sciences STS network Foundation [KFJ-SW-STS-141-01]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA080X0X0X]
- foundation of Innovation team International Partner Program of Chinese Academy of Sciences [KZZD-EW-TZ-07-08]
AimsAbscisic acid (ABA) has been shown to prime rice seedlings for enhanced tolerance to alkaline stress under both greenhouse and field conditions. This study aimed to understand the mechanism of the ABA priming effect.MethodsRice seedlings were grown hydroponically and pretreated with ABA (10M) for 24h, and then subjected to alkaline stress simulated by 15mM Na2CO3 solution (pH 10.87). Paraquat (2.5-25M) was used as an intracellular generator of reactive oxygen species (ROS).ResultsCompared to the control treatment, pretreatment with ABA significantly mitigated root damage and improved the survival rate of rice seedlings under alkaline conditions. Furthermore, ABA pretreatment increased the antioxidant enzyme activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, and reduced the alkalinity- and paraquat-induced ROS accumulation (O-2(-) and H2O2) and seedling injury and mortality. In addition, the expression of the ABA-responsive genes SalT and OsWsi18, as well as the stress tolerance-related genes OsJRL, OsPEX11, OsNAC9, OsAKT1 and OsHKT1, was superinduced by ABA pretreatment under alkaline conditions.ConclusionsABA priming enhances tolerance to alkaline stress by upregulating the antioxidant defense system and stress tolerance-related genes in the roots of rice seedlings.
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