期刊
PLANT AND CELL PHYSIOLOGY
卷 60, 期 5, 页码 1146-1159出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcz031
关键词
Abscisic acid; Arabidopsis; Cytosolic calcium; Reactive carbonyl species; Reactive oxygen species; Stomatal closure
资金
- Japan Society for the Promotion of Science [26440149]
- Grants-in-Aid for Scientific Research [26440149] Funding Source: KAKEN
We have demonstrated that reactive carbonyl species (RCS) function as an intermediate downstream of hydrogen peroxide (H2O2) production in abscisic acid (ABA) signaling for stomatal closure in guard cells using transgenic tobacco plants overexpressing alkenal reductase. We investigated the conversion of the RCS production into downstream signaling events in the guard cells. Both ABA and H2O2 induced production of the RCS, such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), in epidermal tissues of wild-type Arabidopsis thaliana plants. Application of the RCS scavengers, carnosine and pyridoxamine, did not affect the ABA-induced H2O2 production but inhibited the ABA- and H2O2-induced stomatal closure. Both acrolein and HNE induced stomatal closure in a plasma membrane NAD(P)H oxidase mutant atrbohD atrbohF as well as in the wild type, but not in a calcium-dependent kinase mutant cpk6. Acrolein activated plasma membrane Ca2+-permeable cation channels, triggered cytosolic free Ca2+ concentration ([Ca2+](cyt)) elevation, and induced stomatal closure accompanied by depletion of glutathione in the guard cells. These results suggest that RCS production is a signaling event between the ROS production and [Ca2+](cyt) elevation during guard cell ABA signaling.
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