期刊
ANALYTICAL METHODS
卷 8, 期 38, 页码 6895-6902出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay01384h
关键词
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资金
- 973 Program Project of China [2015CB352101]
- Major International Cooperation Project of the Natural Science Foundation of China [61320106002]
- Marine Public Welfare Project of China [201305010]
- Public Welfare Project of Zhejiang Province [2015C34010]
- Natural Science Foundation of China [31228008, 31571004]
Okadaic acid (OA) and saxitoxin (STX) are common marine toxins which can accumulate in shellfish and affect human health through the food chain. A convenient and efficient method is urgently required to perform on-site detection of marine toxins to avoid frequent poisoning incidents. In this work, a smartphone-based system cooperating with competitive immunoassay strips was designed for rapid on-site detection of OA and STX. The smartphone was employed as a light detector for image acquisition and data processing. The 3D-printed portable accessory of the smartphone was utilized to fix the test strips. The homemade APP -iStrip used the pixel scan to obtain the valley value of the pixel curve as the output of the strip. The strip analytical method was easy to operate with a detection limit of 2.800 ng ml(-1) for OA and 9.808 ng ml(-1) for STX. Moreover, with the optimization of the antigen-antibody ratio on the strip, it provided quantitative analysis of OA and STX in the range of 3-20 ng ml (-1) and 10-100 ng ml(-1), respectively. Taking advantage of the smartphone and biochemical analysis, the strip analytical system will be a promising tool for on-site rapid detection of these two toxins.
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