期刊
ANALYTICAL METHODS
卷 8, 期 25, 页码 5001-5008出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay00406g
关键词
-
资金
- Natural Science Foundation of China (NSFC) [61505027, 61535003, 61275182]
- National Key Basic Research Program of China [2015CB352002]
- Natural Science Foundation of Jiangsu Province [BK20150623]
- Excellent Youth Foundation of Jiangsu Province [BK20140023]
- Innovation Fund of School of Electronic Science and Engineering Southeast University [2242015KD002]
- Science Foundation for the Excellent Youth Scholars of Southeast University
- Fundamental Research Funds for the Central Universities
Exosomes play an important role in intercellular communications. Here, we present a surface-enhanced Raman scattering (SERS) based detection method for tumor-derived exosomes using SERS nanoprobes and magnetic nanobeads. The SERS nanoprobe has a core-shell structure, with gold core-silver shell nanorods (Au@Ag NRs) as the SERS active core, Raman molecules as the SERS reporter and a silica layer as the protecting shell. The outmost surface of the SERS nanoprobe is decorated with exosome-specific antibodies. Each magnetic nanobead is fabricated by coating Fe3O4 nanoparticles (NPs) with a silica shell and attaching specific antibodies to the silica shell. With target exosomes present, the magnetic nanobeads and SERS nanoprobes can capture the exosomes by forming a sandwich-type immunocomplex. The immunocomplex (as well as the SERS nanoprobes) can be precipitated with a magnet, and thus SERS signals can be detected in the precipitates. With no target exosomes present, no immunocomplex can be formed, and thus quite weak SERS signals will be detected in the precipitates. Hence, the SERS signal of the final magnetic separation product can be used to detect exosomes. In the experiment, using one kind of tumor cells and one kind of normal cells, we proved that the presented method can be used for both qualitative and quantitative detection of tumor-derived exosomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据