期刊
PEPTIDES
卷 112, 期 -, 页码 114-124出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2018.12.003
关键词
Brainstem; Food intake; Fos; Hypothalamus; Immunohistochemistry; Kisspeptin-10
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan [17H04027, 17K15575]
- UOEH Research Grant for Promotion of Occupational Health from the University of Occupational and Environmental Health, Japan
- Research Project for Improving Quality in Healthcare and Collecting Scientific Evidence on Integrative Medicine from Japan Agency for Medical Research and development (AMED), Japan
- Grants-in-Aid for Scientific Research [17K15575, 17H04027] Funding Source: KAKEN
Kisspeptin (KP), known as a hypothalamic neuropeptide, plays a critical role in the regulation of not only reproduction but also food intake. The anorectic neuropeptides, nesfatin-1 and oxytocin (OXT), are expressed in central nervous system, particulaly in various hypothalamic nuclei, and peripheral tissue. We examined the effects of the intracerebroventricular (icv) administration of KP-10 on feeding and nesfatin-1-immunoreactive (ir) or OXT-ir neurons in the rat hypothalamus, using Fos double immunohistochemistry in male rats. Cumulative food intake was remarkably decreased 0.5-3 h after icy administration of KP-10 (6.0 mu g) compared to the vehicle treated and the KP-10 (3.8 mu g) treated group. The icy administration of KP-10 significantly increased the number of nesfatin-1-ir neurons expressing Fos in the supraoptic nucleus (SON), paraventricular nucleus (PVN), arcuate nucleus (ARC), dorsal raphe nucleus, locus coeruleus, and nucleus tractus solitarius. The decreased food intake induced by KP-10 was significantly attenuated by pretreatment with the icy administration of antisense RNA against nucleobindin-2. After icy administration of KP-10, the percentages of OXT-ir neurons expressing FOS were remarkably higher in the SON and PVN than for vehicle treatment. The KP-10-induced anorexia was partially abolished by pretreatment with OXT receptor antagonist (OXTR-A). The percentage of nesfatin-1-ir neurons expressing Fos-ir in the ARC was also decreased by OXTR-A pretreatment. These results indicate that central administration of KP-10 activates nesfatin-1- and OXT neurons, and may play an important role in the suppression of feeding in male rats.
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