Determination of Bovine Hemoglobin by Near-Infrared Diffuse Reflectance Spectroscopy with Novel Adsorption Preconcentration

A method for the quantitative determination of bovine hemoglobin in dilute solution was developed using adsorption preconcentration and near-infrared diffuse reflectance spectroscopy. An adsorbent, designated as multicarbonyl polymer-grafted silica particles, was prepared for the preconcentration of bovine hemoglobin in dilute solution. Under neutral conditions, the adsorption efficiency exceeded 98% within 20min. After the preconcentration of bovine hemoglobin on the adsorbent, the near-infrared spectrum was measured in diffuse reflectance mode and a partial least squares model was constructed for quantitative prediction. Samples were analyzed in the presence of amino acid, albumin bovine V, D-glucose, and metal ions as potential interferences. The results show that bovine hemoglobin was selectively determined. The correlation coefficient between the predicted concentrations and the reference values was 0.9911, and the recoveries were from 86.4 to 111.2% for validation samples with concentrations between 2.1 and 30.0mgL(-1). Therefore, the determination of bovine hemoglobin was achieved by near-infrared diffuse reflectance spectroscopy combined with preconcentration and chemometric modeling.