期刊
ORGANIC LETTERS
卷 21, 期 7, 页码 2241-2245出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.9b00524
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资金
- National Natural Science Foundation of China [81725023, 81891010/81891011, 21573205]
- Beijing Natural Science Foundation [JQ18027]
- Young Elite Scientists Sponsorship Program by China Association for Science and Technology [2016QNRC001]
A highly regio-specific and donor-promiscuous 3-O-glycosyltransferase, Sb3GT1 (UGT78B4), was discovered from Scutellaria baicalensis. Sb3GT1 could accept five sugar donors (UDP-Glc/-Gal/-GlcNAc/-Xyl/-Ara) to catalyze 3-O-glycosylation of 17 flavonols, and the conversion rates could be >98%. Five new glycosides were obtained by scaled-up enzymatic catalysis. Molecular modeling and site-directed mutagenesis revealed that G15 and P187 were critical catalytic residues for the donor promiscuity. Sb3GT1 could be a promising catalyst to increase structural diversity of flavonoid 3-O-glycosides.
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