期刊
ANALYTICAL CHEMISTRY
卷 88, 期 19, 页码 9691-9698出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02584
关键词
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资金
- National Natural Science Foundation of China [21545005, 31501570, 21375072]
- Natural Science Foundation of Shandong Province, China [ZR2014BQ011]
- Research Foundation for Distinguished Scholars of Qingdao Agricultural University [663-1115003, 663-1113311]
- Special Foundation for Taishan Scholar of Shandong Province [ts201511052]
Herein, a novel universal and label-free homogeneous. electrochemical platform is demonstrated, on which a complete set of DNA-based two-input Boolean logic gates (OR, NAND, AND, NOR, INHIBIT, IMPLICATION; XOR, and XNTOR) is constructed by simply and rationally deploying the designed DNA polymerization/nicking machines without complicated sequence modulation. Single-stranded DNA is employed as the proof-of-concept target/input to initiate or prevent the DNA polymerization/nicking cyclic reactions on these DNA, machines: to synthesize numerous intact G-quadruplex sequences or binary G-quadruplex subunits as the output. The generated output strands then self-assemble into G-quadruplexes that render remarkable decrease to the diffusion current response of methylene blue and, thus; provide the amplified homogeneous electrochemical readout signal net only for the logic gate operations but also for the ultrasensitive detection of:the target/input, This system represents the first example of homogeneous electrochemical logic operation. Importantly, the proposed homogeneous electrochemical logic gates possess the input/output homogeneity and share a constant output threshold value. Moreover, the modular design of DNA polymerization/nicking machines enables the adaptation of these homogeneous electrochemical logic gates to various input and output sequences. The results of this study demonstrate;the versatility and universality of the label free homogeneous: electrochemical platform in the design of biomolecular logic gates and provide a potential platform for :the further development of large-scale DNA-based biocomputing circuits and advanced biosensors for multiple molecular targets.
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