期刊
ANALYTICAL CHEMISTRY
卷 88, 期 19, 页码 9789-9795出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02847
关键词
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资金
- National Natural Science Foundation of China [21435005]
- Development Project of Science and Technology of Jilin Province [20150520011JH]
The emergence of fluorescent nanomaterials with excellent performances has triggered the development Of fluorescence analysis technique, which possesses several advantages in the research and clinical applications. However, current strategies :for fluorescence immunoassay: usually involve tontine fluorophore-labeled antibody and/or awkward signal generation procedure that may not be available in conventional enzyme-linked immunosorbent assay (ELISA) systems. Herein; we circumvent this problem by imparting an exquisite signal, generation mechanism to commercially available alkaline phosphatase (ALP)-based ELISA platform and putting forward a conceptual fluorescent ELISA system based on an original ALP-enabled in situ synthesis of fluorescent nanomaterials. After adding target antigen, the presence of ALP labeled on antibody catalyzes the transformation of the substrate ascorbic acid 2-phosphate into ascorbic acid. Then the resultant ascorbic acid (i.e ascorbate) interacts with amine-containing silane molecules (no fluorescence) to produce intense cyan fluorescent silicon nanoparticles. For the-proof-of-concept, alpha-fetoprotein and human immunoglobulin G are chosen as the model antigen targets, and our proposed immunoassay (designated as the nanoparticles generation -based fluorescent ELISA) enables the detection with either fluorescence spectroscopy or naked-eye readout under the ultraviolet lamp. The convincing recognition mechanism and assay performance ensure fluorescent ELISA to quantitatively evaluate the alpha-fetoprotein level in serologic test and potentially apply in the clinic diagnosis of hepatocellular carcinoma.
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