期刊
ANALYTICAL CHEMISTRY
卷 88, 期 6, 页码 3295-3303出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b04773
关键词
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资金
- NIH [GM080148, AI091646, AI104317]
- National Defense Science & Engineering Graduate Fellowship (NDSEG)
- NSF Graduate Research Fellowship [DGE-1256259]
- NIH Training Fellowship [T32GM008505]
We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectra. Here we demonstrate this approach in cultured cells by monitoring a viral infection and the corresponding viral protein production over many infection time points in a single experiment. In this context :the NeuCode PRM combination affords up to 30 channels of quantitative information in a single MS experiment.
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