期刊
ONCOLOGY REPORTS
卷 41, 期 4, 页码 2126-2136出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2019.7018
关键词
adipose-derived mesenchymal stem cells; tumor tropism; tumorsphere formation; epithelial-mesenchymal transition; breast cancer
类别
资金
- National Natural Science Foundation of China [81471881, 81372083]
- Health Collaborative Innovation major projects of Guangzhou [7414275040815]
- Natural Science Foundation of Guangdong Province of China [2014A030310155]
- Entry Point Project of Guangdong Province of China [PY2014N036]
- Innovative project of Guangdong Province of China [2014KQNCX046]
- Administrator Foundation of Nanfang Hospital [2014B009]
- Key Clinical Specialty Discipline Construction Program
Mesenchymal stem cells reportedly have a marked effect on tumor growth or suppression. However, it remains uncertain whether adipose-derived mesenchymal stem cells (ADSCs) from grafted fat can contribute to breast cancer growth and recurrence. In the present study, interactions between ADSCs and MCF-7 breast cancer cells were evaluated in a Matrigel co-culture system and in an in vivo nude mouse model. Results suggested that MCF-7 cells exerted tumor tropism effects on ADSCs and this may be regulated by chemokines, such as the macrophage inflammatory protein (MIP)-1 delta and MIP-3 alpha. Additionally, ADSCs significantly induced tumorsphere formation in vitro and promoted tumorigenicity in vivo. RT-qPCR analysis indicated that tumorsphere formation by MCF-7 cells was associated with the induction of stem-like properties, which was mediated by epithelial-mesenchymal transition. Together, the present findings indicated that ADSCs exhibit tropism and induce tumorsphere formation of MCF-7 cells.
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