期刊
ANALYTICAL CHEMISTRY
卷 88, 期 4, 页码 2051-2058出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b03109
关键词
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资金
- National Natural Science Foundation of China [21325523, 21405169, 21527811]
- Award for One Hundred Talent Program of Nanyue of Guangdong Province
- Award for Pengcheng Distinguished Scholars of Shenzhen City
- Award for Team Leader Program of Taishan Scholars of Shandong Province, China
Nanomaterial-based differential sensors (e.g., chemical nose) have shown great potential for identification of multiple proteins because of their modulatable recognition and transduction capability but with the limitation of array separation, single-channel read-out, and long incubation time. Here, we develop a multicolor quantum dot (QD)-based multichannel sensing platform for rapid identification of multiple proteins in an array-free format within 1 min. A protein-binding dye of bromophenol blue (BPB) is explored as an efficient reversible quencher of QDs, and the mixture of BPB with multicolor QDs may generate the quenched QD-BPB complexes. The addition of proteins will disrupt the QD-BPB complexes as a result of the competitive protein-BPB binding, inducing the separation of BPB from the QDs and the generation of distinct fluorescence patterns. The multicolor patterns may be collected at a single-wavelength excitation and differentiated by a linear discriminant analysis (LDA). This multichannel sensing platform allows for the discrimination of ten proteins and seven cell lines with the fastest response rate reported to date, holding great promise for rapid and high-throughput medical diagnostics.
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