期刊
NUCLEIC ACIDS RESEARCH
卷 47, 期 8, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz107
关键词
-
资金
- Bellberry-Viertel Senior Medical Research Fellowship
- Australian National Health and Medical Research Council (NHMRC) Fellowship [GNT1104924]
- NHMRC [GNT1098290, GNT1140976]
- Victorian State Government Operational Infrastructure Support
- NHMRC Research Institute Infrastructure Support Scheme
- Walter and Eliza Hall Institute of Medical Research
Systematic variation in the methylation of cytosines at CpG sites plays a critical role in early development of humans and other mammals. Of particular interest are regions of differential methylation between parental alleles, as these often dictate monoallelic gene expression, resulting in parent of origin specific control of the embryonic transcriptome and subsequent development, in a phenomenon known as genomic imprinting. Using long-read nanopore sequencing we show that, with an average genomic coverage of similar to 10, it is possible to determine both the level of methylation of CpG sites and the haplotype from which each read arises. The long-read property is exploited to characterize, using novel methods, both methylation and haplotype for reads that have reduced basecalling precision compared to Sanger sequencing. We validate the analysis both through comparison of nanopore-derived methylation patterns with those from Reduced Representation Bisulfite Sequencing data and through comparison with previously reported data. Our analysis successfully identifies known imprinting control regions (ICRs) as well as some novel differentially methylated regions which, due to their proximity to hitherto unknown monoallelically expressed genes, may represent new ICRs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据