期刊
ANALYTICAL CHEMISTRY
卷 88, 期 11, 页码 6018-6025出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b01141
关键词
-
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Foundation for Innovation (CFI)
The deposition of sodium salts followed by a sputtered layer of gold has been demonstrated to be a power combination for the analysis of triacylglycerols (TAGs) from tissue sections by laser desorption ionization (LDI) imaging mass spectrometry (IMS). Various sodium salts were tested for their capability to ionize TAGs and their ability to produce fast drying, small crystals (<= 3 mu m). The spray deposition of a sodium acetate and carbonate buffer mixture at pH 10.3 on which a 28 +/- 3 nm sputtered layer of gold (Au-CBS) is subsequently deposited was found to provide the most effective combination for TAG analysis by high imaging resolution IMS. Under these conditions, a 30-fold increase in TAG signal intensity was observed when compared to matrix assisted LDI (MALDI) methods using 2,5-dihydrobenzoic acid as matrix. Furthermore, Au-CBS led to an increase in the number of detected TAG species from similar to 7 with DHB to more than 25 with the novel method, while few phospholipid signals were observed. These results were derived from the IMS investigation of fresh frozen mouse liver and rabbit adrenal gland tissue sections with a range of higher spatial resolutions between 35 and 10 mu m. Au-CBS-LDI MS presents a highly sensitive and specific alternative to MALDI MS for imaging of TAGs from tissue sections. This novel approach has the potential to provide new biological insights on the role of TAGs in both health and disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据