期刊
ANALYTICAL CHEMISTRY
卷 88, 期 7, 页码 3686-3696出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b04472
关键词
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资金
- National Institutes of Health [R21HG006278, P41EB020594]
- National Science Foundation [1507577]
- Ulsan National Institute of Science and Technology [1.130090.01]
- Roche
- National Science Foundation
- ACS Division of Analytical Chemistry
- Society for Analytical Chemists of Pittsburgh
We present a novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution. Thermoplastic surfaces were activated by UV/O-3 or O-2 plasma treatment under various conditions to generate pendant surface-confined carboxylic acids (-COOH). These surface functional groups were then labeled with a photoswitchable dye and interrogated using single-molecule, localization-based, super-resolution fluorescence microscopy to elucidate the surface heterogeneity of these functional groups across the activated surface. Data indicated nonuniform distributions of these functional groups for both COC and PMMA thermoplastics with the degree of heterogeneity being dose dependent. In addition, COC demonstrated relative higher surface density of functional groups compared to PMMA for both UV/O-3 and O-2 plasma treatment. The spatial distribution of -COOH groups secured from super-resolution imaging were used to, simulate nonuniform patterns of electroosmotic flow in theilhoplastic nanochannels. Simulations were compared to single-particle tracking of fluorescent nanoparticles within thermoplastic nanoslits to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process.
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