期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 26, 期 3, 页码 220-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41594-019-0193-2
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资金
- National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 OD010440]
- Office of the Director, NIH (OD) of the National Institutes of Health [S10OD016374]
- NIH [R37 HD37047]
- National Science Foundation [DGE-1746891]
- [T32 GM007445]
RNA granules are subcellular compartments that are proposed to form by liquid-liquid phase separation (LLPS), a thermodynamic process that partitions molecules between dilute liquid phases and condensed liquid phases. The mechanisms that localize liquid phases in cells, however, are not fully understood. P granules are RNA granules that form in the posterior of Caenorhabditis elegans embryos. Theoretical studies have suggested that spontaneous LLPS of the RNA-binding protein PGL-3 with RNA drives the assembly of P granules. We find that the PGL-3 phase is intrinsically labile and requires a second phase for stabilization in embryos. The second phase is formed by gel-like assemblies of the disordered protein MEG-3 that associate with liquid PGL-3 droplets in the embryo posterior. Co-assembly of gel phases and liquid phases confers local stability and long-range dynamics, both of which contribute to localized assembly of P granules. Our findings suggest that condensation of RNA granules can be regulated spatially by gel-like polymers that stimulate LLPS locally in the cytoplasm.
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