期刊
NATURE NANOTECHNOLOGY
卷 14, 期 5, 页码 447-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41565-019-0375-4
关键词
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资金
- National Research Foundation (NRF), Prime Minister's Office, Singapore under its Campus for Research Excellence and Technological Enterprise (CREATE) program
- Sime Darby Malaysia
- Agency of Science, Research and Technology, Singapore
- Swiss National Science Foundation [P300P2_174469]
- Swiss National Science Foundation (SNF) [P300P2_174469] Funding Source: Swiss National Science Foundation (SNF)
Plant genetic engineering is an important tool used in current efforts in crop improvement, pharmaceutical product biosynthesis and sustainable agriculture. However, conventional genetic engineering techniques target the nuclear genome, prompting concerns about the proliferation of foreign genes to weedy relatives. Chloroplast transformation does not have this limitation, since the plastid genome is maternally inherited in most plants, motivating the need for organelle-specific and selective nanocarriers. Here, we rationally designed chitosan-complexed single-walled carbon nanotubes, utilizing the lipid exchange envelope penetration mechanism. The single-walled carbon nanotubes selectively deliver plasmid DNA to chloroplasts of different plant species without external biolistic or chemical aid. We demonstrate chloroplast-targeted transgene delivery and transient expression in mature Erucasativa, Nasturtium offidnale, Nicotianatabacum and Spinaciaoleracea plants and in isolated Arabidopsisthaliana mesophyll protoplasts. This nanoparticle-mediated chloroplast transgene delivery tool provides practical advantages over current delivery techniques as a potential transformation method for mature plants to benefit plant bioengineering and biological studies.
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