期刊
NATURE METHODS
卷 16, 期 3, 页码 263-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-019-0326-x
关键词
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资金
- Koch Institute Support Grant from the National Cancer Institute [P30-CA14051]
- Ludwig Center for Molecular Oncology
- Cancer Systems Biology Consortium from the NCI [U54 CA217377]
- Institute for Collaborative Biotechnologies from the US Army Research Office [W911NF-09-0001]
- Wellcome Trust (Sir Henry Postdoctoral Fellowship) [110275/Z/15/Z]
- Samsung scholarship
- Wellcome Trust [110275/Z/15/Z] Funding Source: Wellcome Trust
The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of <1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of <15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics.
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